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大鼠热射病发病早期免疫状态的观察
作者:周京江 宋青 赵佳佳 胡婕 杨文超 
单位:221000  江苏徐州 徐州医科大学附属医院急诊科(周京江) 100853  北京 解放军总医院第一医学中心重症医学科(宋青、赵佳佳、胡婕、杨文超) 
关键词:热射病 调节性T细胞 细胞凋亡 
分类号:R594.12
出版年,卷(期):页码:2019,44(10):817-822
摘要:

 [摘要]  目的  观察大鼠热射病早期的免疫状态。方法  成年雄性SD大鼠140只,随机分为对照组(n=20)、经典型热射病模型组(CHS组,n=60)和劳力性热射病模型组(EHS组,n=60);后两组按取样时间点再分为6个亚组(造模成功后0612244872 h),每亚组10只。分别构建CHSEHS大鼠模型,流式细胞术检测造模后不同时间点大鼠外周血和脾脏CD4+CD25+Foxp3+调节性T细胞(Tregs)百分比及脾脏Tregs凋亡率。结果  CHS组大鼠外周血及脾脏Tregs 百分比随时间推移逐渐增高,前24 h低于对照组,24 h之后高于对照组(P<0.05)EHS组大鼠外周血及脾脏Tregs百分比随时间推移逐渐降低,外周血Tregs百分比在0 h略高于对照组,之后均低于对照组,脾脏Tregs百分比在前12 h高于对照组,24 h后低于对照组(P<0.05)。随着时间的延长,CHS组大鼠脾脏Tregs凋亡率呈逐渐下降的趋势,前12 h略高于对照组,24 h后低于对照组(P<0.05)EHS组大鼠脾脏Tregs凋亡率呈逐渐增高的趋势,各时间点均高于对照组(P<0.05)结论  CHS大鼠发病早期Tregs比例呈升高趋势,处于免疫抑制状态;EHS大鼠发病早期Tregs比例呈降低趋势,可能与EHS早期过度激活的炎症反应有关。

 [Abstract]  Objective  To better understand the immune status in the rats after heatstroke. Methods  Adult male SD rats were randomized into 3 groups: control group (n=20), classic heatstroke group (CHS, n=60) and exertional heatstroke group (EHS, n=60). Flow cytometry was employed to detect the percentage of CD4+CD25+Foxp3+ regulatory T cells (Tregs) in arterial blood and spleen as well as the apoptosis rate of Tregs in the spleen. To trace the dynamic changes on Treg cells, samples were collected at specific time points after heatstroke modeling (0, 6, 12, 24, 48, 72 hours post heatstroke). Results  The proportion of Tregs in artery blood and spleen in CHS group increased gradually with a percentage lower than those in control group at first 24 h but higher than those in control group after 24 h. Interestingly, the proportion of Tregs in artery blood and spleen in EHS group was gradually reduced over time. The Tregs in the artery blood slightly higher than that in control group at 0 h followed by less than that in control group afterward; while Tregs in the spleen were higher than that in control group within 12 h, followed by less than that in control group after 24 h. The proportion of apoptotic Tregs in the spleen in CHS group was gradual decline over time, in the first 12 h they were higher than that in control group but lower than that in control group after 24 h. On the contrary, the proportion of apoptotic Tregs in the spleen in EHS group was gradually increased over time and was higher than that in control group at all time points. Conclusions  The proportion of Tregs in peripheral blood and spleen in CHS group showed a rising trend during the early period. This is consistent with the trend in the spleen Tregs apoptosis rate, indicated that immunosuppression may have appeared in the early onset of CHS rats. However, the proportion of Tregs in peripheral blood and spleen in EHS group showed a trend of gradual reduction which is consistent with the spleen Tregs apoptosis rate. The opposite phenotype detected from CHS and EHS rats may due to the excessive inflammatory reaction promoted the apoptosis of Tregs in EHS rats.

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国家自然科学基金(81501642)
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