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不同辐射剂量诱导的犬急性放射性肠损伤模型的建立及其组织学评估
作者:许文达 李宏宇 陈江 郭晓钟 
单位:075000  河北张家口 陆军第八十一集团军医院(原解放军第251医院)消化内科(许文达) 110016  沈阳 北部战区总医院消化内科(李宏宇、陈江、郭晓钟) 
关键词:急性放射性肠损伤 动物模型  
分类号:R574
出版年,卷(期):页码:2019,44(2):107-112
摘要:

[摘要]  目的  建立不同辐射剂量诱导的犬急性放射性肠损伤模型,并对其损伤程度进行组织学评估。方法  将41只健康比格犬随机分为11个不同剂量辐射组(810121416182022242630Gy,每组3)及对照组(8)。各辐射组采用调强照射的方式,进行单次腹部照射。采用HE染色、透射电镜、扫描电镜及组织病理评分等对辐射造成的肠道损伤程度进行评估。结果  HE染色法及透射、扫描电子显微镜观察发现,随着辐射剂量的增加,肠道组织损伤加重。8Gy组呈现轻度损伤,表现为轻度黏膜改变,肠绒毛部分缺失,上皮细胞凋亡或坏死;10~14Gy组呈中度损伤,表现为部分黏膜损伤,肠绒毛脱落,腺体扩张,炎性细胞浸润,血管充血、出血;16~30Gy组呈重度损伤, 表现为弥漫性肠坏死、糜烂、剥脱,广泛的充血及出血等。结论  成功建立不同辐射剂量诱导的犬急性放射性肠损伤模型;该模型的建立及其组织学评估方法可以为新型抗辐射药物的研制提供平台及实验参考。

[Abstract]  Objective  To evaluate acute intestinal injury models induced by radiation through histological analysis. Methods  A total of 41 Beagle canines were randomized into control group (n=8) and 11 radiation groups (at dose of 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 30Gy, 3 each group). Animals were given single-dose from X-ray delivered at dose rates of 250cGy/min using tridimensional conformal radiotherapy (3D-CRT) on the abdomen, followed by histological analysis on the intestine. Results   We successfully developed canine acute intestinal injury model using irradiation. The intestinal mucosal injury showed a dose-dependent manner. The greater radiation dose was received, the more severe pathological mucosal injury observed. For dogs that received 8Gy, their small intestine exhibited a slight grade of apoptosis and partial loss of the intestinal villi in the epithelial cells. For dogs that received moderate irradiation dose of 10-14Gy, we observed partially damaged mucosa, glandular dilatation, inflammatory infiltration, vascular congestion, and hemorrhage in their intestine tissue. For dogs that received the high dose of 16-30Gy, their intestine histologic changes included diffuse intestinal necrosis, erosions or exfoliation, as well as extensive congestion and bleeding. Conclusions  A canine model of acute intestinal injury induced by irradiation in a dose-dependent manner was successfully established. This model would pave the foundation for better irradiation model development and be beneficial to develop novel and effective radioprotective agents.

基金项目:
辽宁省自然科学基金(20170540949)
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