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杞藿饮对湿热环境睡眠剥夺大鼠肝脏的保护作用
作者:王剑 李东涛 阿古拉 陈佳钰 王浩婷 黄文 封颖璐 
单位:266021  山东青岛 青岛大学医学部中西医结合中心(王剑) 266071  山东青岛 海军青岛特勤疗养中心第二疗养区(王剑、李东涛、阿古拉、陈佳钰、王浩婷) 200433  上海 海军军医大学附属长海医院野战内科学研究所(黄文) 266071  山东青岛 海军第971医院中医科(封颖璐) 
关键词:杞藿饮 睡眠剥夺 湿热环境 疲劳 肝组织 中医药 
分类号:R8.243
出版年,卷(期):页码:2019,44(1):20-25
摘要:

[摘要]  目的  探讨杞藿饮(QHD)对湿热环境下睡眠剥夺大鼠肝脏的保护作用。方法  40只雄性SD大鼠采用随机数字表法分为对照组(BC)、湿热环境对照组(HC)、湿热环境睡眠剥夺组(HS)、湿热环境睡眠剥夺QHD(HSQ)4组,每组10只。BC组大鼠常温常湿下饲养,HC组、HS组、HSQ组大鼠模拟湿热环境下饲养。BC组、HC 组、HS组大鼠给予蒸馏水灌胃,HSQ组大鼠给予QHD灌胃,连续15dHS组、HSQ组大鼠予以72h睡眠剥夺。检测各组大鼠血清丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)水平,肝组织中肝糖原、丙二醛(MDA)和还原型谷胱甘肽(GSH)水平。比较各组大鼠β-半乳糖苷酶(β-gal)在肝细胞中的表达及其超微结构变化。结果  4组大鼠血清AST水平依次为(107.7±8.4)U/L(126.9±10.4)U/L(176.9±13.1)U/L(140.6±9.6)U/LALT水平依次为(37.9±3.1) U/L(45.5±6.2)U/L(86.5±10.4)U/L(64.9±8.7)U/L,组间比较差异均有统计学意义(P<0.01);与BC组比较, HC组、HS组、HSQ组大鼠血清ASTALT均明显升高(P<0.01)HSQ组大鼠血清ASTALT明显低于HS(P<0.01)4组大鼠肝组织中肝糖原水平依次为:(19.7±1.5)nmol/mg prot(17.9±0.8)nmol/mg prot(11.3±0.5)nmol/mg prot(14.6±0.7)nmol/mg protMDA水平依次为(10.3±1.1)nmol/mg prot(26.6±1.3)nmol/mg prot(41.4±2.4)nmol/mg prot(33.7±1.7)nmol/mg protGSH水平依次为:(77.6±5.2)U/mg prot(70.6±5.1)U/mg prot(45.8±3.8)U/mg prot(60.7±3.3)U/mg prot,组间比较差异均有统计学意义(P<0.01);与BC组比较,HC组、HS组、HSQ组大鼠肝脏组织中肝糖原和GSH含量明显减少(P<0.05)MDA含量明显增加(P<0.01);与HS组比较,HSQ组大鼠肝组织中肝糖原和GSH含量明显增加(P<0.01)MDA含量明显减少(P<0.01)4组大鼠肝组织β-gal阳性细胞计数值依次为0.67±0.526.23±0.4213.5±0.4810.16±0.36,组间比较差异有统计学意义(P<0.01)HC组、HS组、HSQ组大鼠的肝组织中β-gal阳性细胞计数值均明显高于BC组大鼠(P<0.01)HSQβ-gal阳性细胞计数值则明显低于HS(P<0.01)。光镜及透射电镜观察HC组、HS组、HSQ组大鼠肝脏均有不同程度的损伤,以HS组大鼠最明显,HSQ组损害有一定程度的减轻。结论  QHD能明显降低湿热环境睡眠剥夺所致的大鼠肝脏损害程度,可能与增加肝糖原储备、平衡氧化应激及减缓肝细胞衰老进程有关。

[Abstract]  Objective  To explore the protective effect of Qihuo decoction (QHD) on the livers of sleep-deprived rats housed in the humid and hot environment, and to characterize ultrastructural changes of livers in these rats. Methods  Forty male SD rats were randomized into 4 groups (10 each group): blank control group (BC group), humid and hot control group (HC group), humid and hot with sleep deprivation group (HS group), and humid and hot with sleep-deprived treated with QHD group (HSQ group). Rats in BC group were housed in the normal temperature and humidity chamber; while rats in HC group, HS group, and HSQ group were housed in the simulated hot and humid chamber. Rats in BC group, HC group, and HS group were given distilled water to gavage for 15 days, and rats in HSQ group were given QHD to gavage for 15 days. Then rats in HS group and HSQ group were sleep deprived for 72 hours. The serum concentrations of alanine aminotransferase (ALT) and serum aspartate aminotransferase (AST) were detected. The liver tissues contents of hepatic glycogen, malondialdehyde (MDA) and reduced glutathione (GSH) were detected. The expression of β-galactosidase (β-gal) in each group was also detected. Lastly, the rat hepatocytes ultrastructure was observed by a transmission electron microscope. Results  In BC group, HC group, HS group, and HSQ group, the serum AST levels were (107.7±8.4)U/L, (126.9±10.4)U/L, (176.9±13.1)U/L, and (140.6±9.6)U/L; the ALT levels were (37.9±3.1)U/L, (45.5±6.2)U/L, (86.5±10.4)U/L, and (64.9±8.7)U/L. Statistical analysis among groups were significantly different (P<0.01). Compared with BC group, the levels of AST and ALT increased significantly in the rest 3 groups (P<0.01). The serum concentrations of AST and ALT in HSQ group were significantly lower than those in HS group (P<0.01). The hepatic glycogen levels were (19.7±1.5)nmol/mg prot, (17.9±0.8) nmol/mg prot, (11.3±0.5)nmol/mg prot, (14.6±0.7)nmol/ mg prot successively in the four groups. The MDA levels were (10.3±1.1)nmol/mg prot, (26.6±1.3)nmol/mg prot, (41.4±2.4) nmol/mg prot, (33.7±1.7)nmol/mg prot successively in the four groups. The GSH levels were (77.6±5.2)U/mg prot, (70.6±5.1) U/mg prot, (45.8±3.8)U/mg prot, (60.7±3.3)U/mg prot successively in the four groups. Comparisons among groups were significantly different (P<0.01). Compared with BC group, the data of HC group, HS group and HSQ group about the contents of hepatic glycogen and GSH decreased significantly (P<0.05), the contents of MDA increased significantly (P<0.01). Compared with HS group, the data of HSQ group about the contents of hepatic glycogen and GSH increased significantly (P<0.01), the contents of MDA decreased significantly (P<0.01). The counts of β-gal positive cells were (0.67±0.52), (6.23±0.42), (13.5±0.48) and (10.16±0.36) successively in rats liver tissues of the four groups. Comparison among groups was significantly different (P<0.01). Compared with the BC group, the data of the HC group, HS group, and HSQ group about the number of β-gal positive cells increased significantly (P<0.01). The number of β-gal positive cells in HSQ group was lower than that in HS group (P<0.01). Light microscopy and transmission electron microscopy showed that the hepatocytes of HC group, HS group and HSQ group were damaged to varying degrees, and the injury of HS group was the most obvious, and the injury of HSQ group was reduced. Conclusion  QHD Can obviously alleviate the degree of liver injury that was induced by high temperature and high humidity and sleep deprivation, and the mechanism of which might be related to increasing hepatic glycogen reserve, balancing oxidative stress and slowing process of hepatic cell aging.

基金项目:
海军后勤保障部科研计划项目(CJN14L078)
作者简介:
王剑,主治医师。主要从事疲劳中医药干预、中医药肿瘤防治研究
参考文献:
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