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外周血淋巴细胞mTOR表达水平与冠状动脉狭窄程度的相关性分析
作者:韦舒杰 马双陶 张彦 王强 李德 杨大春 杨永健 
单位:400042 重庆 第三军医大学研究生管理大队(韦舒杰) 610083 成都 成都军区总医院心血管内科(韦舒杰、马双陶、张彦、王强、李德、杨大春、杨永健) 
关键词:冠状动脉疾病 雷帕霉素靶蛋白 淋巴细胞 
分类号:R541.4
出版年,卷(期):页码:2016,41(3):238-242
摘要:
[摘要] 目的 探讨外周血淋巴细胞中雷帕霉素靶蛋白(mTOR)表达水平与冠状动脉狭窄程度的相关性。方法 连续纳入170例在成都军区总医院心血管内科接受选择性冠脉造影的患者,其中男86例,女84例,年龄18~82岁,根据造影结果分为冠脉正常组和冠脉病变组,并计算冠脉病变的Gensini积分。采集两组患者的年龄、性别、民族、身高和体重等资料,测定空腹血糖、血脂及肝肾功能等生化指标,提取外周血淋巴细胞总RNA,采用Real time qPCR技术测定mTOR表达水平,分析mTOR表达水平及其他心血管危险因素与Gensini积分的相关性。结果 在170例患者中,冠脉病变组的年龄、体重指数、甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白(LDL-C)以及外周血淋巴细胞mTOR表达水平显著高于冠脉正常组,差异均有统计学意义(P<0.05)。相关分析结果显示,外周血淋巴细胞mTOR表达水平、TGTC和年龄与冠脉病变狭窄程度Gensini积分呈正相关,相关系数r分别为0.3430.2540.2360.207(P<0.01)结论 外周血淋巴细胞mTOR表达水平与冠脉病变狭窄程度呈正相关,提示淋巴细胞mTOR在冠脉病变发展过程中可能发挥重要作用。

[Abstract]  Objective  To investigate the correlation of the expression level of mammalian target of rapamycin (mTOR) gene in peripheral blood lymphocyte with the degree of stenosis of the coronary artery. Methods  One hundred and seventy consecutive patients (male 86, female 84, aged from 18 to 82) who received coronary angiography (CAG) in the General Hospital of Chengdu Command were divided into coronary stenosis group and coronary normal group according to the result of CAG, and the Gensini scores were calculated based on the result of selective CAG. For the two groups, the age, gender, ethnic group, height and weight were collected, and the fasting glucose, lipids, liver and renal function parameters were measured, the total RNA was extracted from peripheral blood lymphocytes, and the mTOR expression level was measured, the correlation between mTOR expression level and other cardiovascular risk factors with Gensini score was then analyzed. Results  Among the 170 patients, the age, body mass index, triglycerides (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C) and the expression level of mTOR gene in peripheral blood lymphocyte were significantly higher in coronary stenosis group than in normal group (P<0.05). Positive correlation was found between the Gensini score with the age, the levels of plasma TG and TC, and the expressions level of mTOR gene, and the correlation coefficients (r) were 0.207, 0.254, 0.236 and 0.343, respectively (P<0.01). Conclusions  The mTOR expression level in peripheral blood lymphocytosis is positively correlated with the degree of coronary artery stenosis, implying that the monocyte mTOR may play an important role in the generation and development of coronary atherosclerosis.

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