网站首页杂志简介编委会成员过刊目录投稿指南在线订阅联系我们读者论坛Mil Med Res
消息通知:

 

位置:首页 >> 在线订阅
表没食子儿茶素没食子酸酯对肝癌细胞增殖的抑制作用研究
作者:王帅 尚志梅 黄利敏 刘亮 武中林 赵阳 祝敬燕 
单位:261041 山东潍坊 潍坊市中医院肿瘤三科(王帅、尚志梅、黄利敏、祝敬燕) 050011 石家庄 河北医科大学第四医院肿瘤所(刘亮、武中林、赵阳) 
关键词:HepG2细胞 没食子酸丙酯 细胞凋亡 细胞增殖 
分类号:R735.7
出版年,卷(期):页码:2016,41(3):199-203
摘要:
[摘要] 目的 探讨表没食子儿茶素没食子酸酯(EGCG)对肝癌细胞HepG2增殖和凋亡的影响及其可能机制。方法 采用不同浓度(02550100200400mg/L)EGCG作用于HepG2细胞,于2448h后采用MTT方法检测细胞增殖抑制率。以不同浓度(050100200mg/L)EGCG作用于HepG2细胞,24h后采用流式细胞术检测细胞凋亡率、细胞周期、细胞分裂周期蛋白25A(CDC25A)Smad3蛋白的表达,RT-PCR检测CDC25ASmad3 mRNA的表达。结果 MTT检测结果显示,不同浓度EGCGHepG2细胞均有生长抑制作用,且具有时间和剂量依赖性(P<0.01)。随着EGCG浓度升高,细胞增殖指数(PI)明显降低(P<0.01),细胞凋亡率明显增高(P<0.01)CDC25A蛋白和mRNA表达水平下降(P<0.05)Smad3蛋白和mRNA表达水平上升(P<0.05)结论 EGCG可能通过下调CDC25A、上调Smad3的表达,抑制HepG2细胞增殖并诱导其凋亡,从而对肝癌细胞起到生长抑制作用。

[Abstract]  Objective  To study the effect of epigallocatechin-3-gallate (EGCG) on the proliferation and apoptosis of hepatic carcinoma HepG2 cells, and to explore the possible mechanism. Methods  HepG2 cells were treated with EGCG in various concentrations (0, 25, 50, 100, 200, 400mg/L) for 24, 48 and 72h, and then the cell proliferation inhibition rate was determined with MTT. Again, the HepG2 cells were treated with various concentrations of EGCG (0, 50, 100, 200mg/L) for 24h, and then the cell apoptosis rate, cell cycle, and the expressions of cell division cycle protein 25A (CDC25A), and Smad3 protein were determined with flow cytometry. mRNA expressions of CDC25A and Smad3 were assessed with RT-PCR. Results  The results of MTT showed that various concentrations of EGCG inhibited the growth of HepG2 cells in dose and time dependent manner (P<0.01). The result of flow cytometry showed that, with the increase of of EGCG concentration, the cell proliferation index (PI) decreased significantly (P<0.01), while the apoptosis rate increased obviously (P<0.01); the expression levels of CDC25A protein and mRNA decreased (P<0.05) and of Smad3 protein and mRNA increased (P<0.05). Conclusion  EGCG may play a role in growth inhibition of hepatic carcinoma cells by down-regulating the expression of CDC25A, up-regulating the expression of Smad3, thus inhibiting proliferation and inducing apoptosis of HepG2 cells.

基金项目:
作者简介:
参考文献:
服务与反馈:
文章下载】【加入收藏

copyright ©《解放军医学杂志》编辑部
地址:北京市海淀区复兴路22号甲3号人民军医出版社   邮编:100036
电话:0201-882929-8021(军线)    010-51927306 (传真)
京ICP备06014771-2