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MicroRNA-124对人脐静脉内皮细胞增殖和凋亡的影响
作者:刘丹 高乃婧 田孝祥 张效林 闫承慧 韩雅玲 
单位:110016  沈阳 沈阳军区总医院心内科 全军心血管病研究所心内科(刘丹、高乃婧、田孝祥、张效林、闫承慧、韩雅玲) 
关键词:微RNAs 动脉粥样硬化 人脐静脉内皮细胞 细胞增殖 细胞凋亡 
分类号:R543.3
出版年,卷(期):页码:2016,41(1):7-11
摘要:

[摘要] 目的 观察miR-124对内皮细胞增殖和凋亡的影响,探讨miR-124在动脉粥样硬化中的作用。方法 采用荧光定量PCR检测miR-12440例冠心病患者及40例正常对照血浆中的表达水平,CCK-8BrdU法检测miR-124对人脐静脉内皮细胞(HUVECs)增殖的影响,TUNNEL染色检测miR-124HUVECs凋亡的影响,Western blotting检测miR-124HUVECs促凋亡蛋白cleaved caspase 3及抗凋亡蛋白Bcl-2表达的影响。结果 与对照组比较,miR-124在冠心病患者血浆中的表达明显降低。CCK-8BrdU检测结果提示miR-124可明显促进HUVECs增殖;TUNNEL染色及Western blotting检测结果提示,miR-124可明显降低HUVECs凋亡细胞的比例及cleaved caspase 3表达,同时增加Bcl-2的表达。结论 MiR-124可促进HUVECs的增殖并抑制其凋亡,为深入认识动脉粥样硬化的发病机制提供了理论依据。

[Abstract]  Objective  To observe the effects of miR-124 on the proliferation and apoptosis of endothelial cells of umbilical cord vein in order to explore the role of miR-124 in the pathogenesis of atherosclerosis. Methods  The expression levels of miR124 in plasma of 40 patients with coronary heart disease and 40 healthy control participants were examined using quantitative RTPCR. CCK8 assay and BrdU incorporation assay were used to analyze the effects of miR-124 on the proliferation of human umbilical vein endothelial cells (HUVECs). The effect of miR-124 on HUVEC apoptosis was examined by TUNNEL staining. The effects of miR-124 on the expression of proapoptotic protein cleaved caspase 3 and anti-apoptotic protein Bcl-2 in HUVECs were examined by Western blotting. Results  Compared with healthy controls, plasma miR-124 was down-regulated in patients with coronary artery disease. In vitro experiment, miR-124 significantly promoted HUVEC proliferation, and it also inhibited HUVECs apoptosis by affecting the expressions of cleaved caspase 3 and Bcl-2. Conclusions  MiR-124 could promote the proliferation and inhibit the apoptosis of HUVECs. This may provide a theoretical basis for clarifying the mechanisms of atherosclerosis.

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