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乌司他丁对热打击血管内皮细胞增殖及炎症因子释放的影响
作者:潘志国 邵玉 耿焱 陈镜合 苏磊 
单位:510010 广州 广州军区广州总医院重症医学科、全军热区创伤救治与组织修复重点实验室(潘志国、耿焱、苏磊) 华侨科(邵玉) 510405 广州 广州中医药大学第一附属医院(陈镜合) 
关键词:乌司他丁 中暑 内皮细胞 细胞增殖 
分类号:R594.12
出版年,卷(期):页码:2015,40(5):362-365
摘要:

目的 探讨乌司他丁对热打击血管内皮细胞增殖及炎症因子释放的影响。方法 将人脐静脉内皮细胞株(HUVEC)细胞分为对照组及不同剂量(1000200030004000500060007000U/ml)乌司他丁组,采用CCK-8法检测不同剂量乌司他丁对热打击下HUEVC细胞增殖的影响。另将HUVEC分为对照组、43热打击组及43热打击+乌司他丁组,采用ELISA法测定乌司他丁对热打击条件下HUVEC细胞炎症因子白介素6(IL-6)和肿瘤坏死因子α(TNF-α)释放的影响。结果 不同剂量乌司他丁对HUVEC细胞增殖均无明显抑制作用(P>0.05)ELISA检测结果显示,培养后即刻(0h)43热打击+乌司他丁组IL-6TNF-α的释放较对照组及43热打击组明显降低(P0.05)6h43热打击+乌司他丁组IL-6TNF-α的释放较43热打击组仍明显降低(P0.05)。结论 乌司他丁对热打击下的HUEVC 细胞增殖无抑制作用,但可抑制细胞IL-6TNF-α的释放。

Objective To explore the influence of ulinastatin on the proliferation of vascular endothelial cells and release of IL-6 and TNF-α under heat stress. Methods HUVEC cells were divided into control, and ulinastatin of different dosage groups (1000, 2000, 3000, 4000, 5000, 6000, 7000U/ml), and CCK-8 assay was used to investigate the influence of ulinastatin on the proliferation of vascular endothelial cells. HUVEC cells were also divided into control, 43 heat stress, and 43 heat stress plus ulinastatin groups. ELISA assay was used to determine the effect of ulinastatin on the release of IL-6 and TNF-α. Results All the above mentioned doses of ulinastatin showed no significant inhibitory effect on proliferation of HUVEC cells (P>0.05). ELISA results showed that, compared with control group and 43 heat-stress group, both the IL-6 and TNF-α releases were decreased at 0h in treatment group with ulinastatin (P<0.05). The IL-6 and TNF-α releases were much lower at 6h in the treatment group with ulinastatin than those undergoing 43 heat-stress only group (P<0.05). Conclusions Various doses of ulinastatin have no effect on proliferation of endothelial cells. However, it can inhibit the IL-6 and TNF-α releases from HUEVC cells under heat stress.

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